Journal: International journal of molecular sciences
Article Title: The Antidepressant Effect of Resveratrol Is Related to Neuroplasticity Mediated by the ELAVL4- Bdnf mRNA Pathway.
doi: 10.3390/ijms26031113
Figure Lengend Snippet: Figure 4. Bdnf as a potential downstream target of ELAVL4: (A) Venn diagram showing the overlap between ELAVL4 target mRNAs and neuroplasticity-related genes. (B,C) Interaction network of the top 100 interactions of ELAVL4 or Bdnf mRNA. (D) AlphaFold3-predicted structural interaction between ELAVL4 protein and Bdnf mRNA. (E) Relative Bdnf expression/Gapdh mRNA expres- sion among the four groups. (F,G) Relative expression of BDNF normalized to GAPDH (N = 3). (H–M) Representative immunofluorescence images showing ELAVL4 and BDNF expression in the DG and CA3 region of the hippocampus (N = 3). Scale bars represent 75 µm. Statistical analyses are performed using one-way ANOVA with Tukey’s post hoc test. (* p < 0.05, ** p < 0.01, and *** p < 0.001).
Article Snippet: The samples were then incubated in 5% BSA (4240GR100, BioFroxx, Bavaria, Germany) for 1 h and subsequently treated with primary mouse anti-ELAVL4 antibody (dilution 1:1000, 67835-1-lg, Proteintech, Chicago, IL, USA), and rabbit anti-BDNF antibody (dilution 1:1000, ER130915, Huabio, Hangzhou, China) overnight at 4 ◦C.
Techniques: Expressing, Immunofluorescence